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. 2011 Aug 22;6(8):e23511. doi: 10.1371/journal.pone.0023511

Figure 6. Bacterial expression of some deletion mutations (DM) from F2 plants.

Figure 6

P1: Shinchunaga wheat; P2: Qinling rye; IPTG: E. coli culture with isopropyl β-Δ-thiogalactopyranoside; CK: E. coli culture without IPTG; arrowhead: the new HMW subunit extracted from F2 seed A-28-22-1; tailed arrowhead: the expressed mature protein from E. coli culture. The sequences used for expression were HQ613181 (DM1), HQ613197 (DM13*), HQ613222 (DM13**), HQ613188 (DM7), HQ613195 (DM11), HQ613220 (DM31), HQ613190 (DM9), HQ613199 (DM15), HQ613196 (DM12), and HQ613218 (DM29).