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. 2011 Aug 22;6(8):e23174. doi: 10.1371/journal.pone.0023174

Figure 2. Testing three different DNA-binding fluorescent dyes for multiplex performance.

Figure 2

EvaGreen (blue), SybrGreen (green) and Syto-13 (red) were tested for peak amplitude and peak separability using the AMELxy multiplex setup. Highest performance in respect to these two criteria was obtained with the EvaGreen dye, as can be seen by the 3–20 times higher melting peaks of the individual amplicons within the multiplex reaction compared to classical SybrGreen. NTC: baseline fluorescence of EvaGreen without DNA template.