Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) A stably transduced shRNA targeting SMURF1 leads to reduced SMURF1 levels (by Western blot) compared to a non-targeting control shRNA. Residual SMURF1 levels, here normalized to GAPDH, are indicated. (B) Stable SMURF1 knockdown reduces anchorage-independent growth (i.e. soft agar colonies). Assay done in triplicate (mean +/− 1SD shown); *, P<0.05 (Student's t-test).
