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. 2011 Aug 22;6(8):e23835. doi: 10.1371/journal.pone.0023835

Figure 7. Inhibition of NHE with EIPA prevents DCA-mediated tissue damage and induction of apoptosis in rat ileal epithelium.

Figure 7

A) Representative H&E staining of rat ileum treated for 2 hours with or without 0.4 mM DCA in the presence or absence of 20 uM EIPA. B) Western blot of active (cleaved) caspase-3 from homogenized tissue treated for 2 hours with or without 0.4 mM DCA in the presence or absence of 20 uM EIPA. Panel C shows representative immunohistochemical staining of active (cleaved) caspase-3 following the same experimental treatment. The brown color brown indicates active caspase-3.