Figure 2. Dox-induced repression of 19 essential yeast genes enhances BMV RNA replication.
Total RNA extracts were obtained from wild type R1158 and untreated and dox-treated (10 µg/ml) essential yeast strains expressing BMV 1a, 2aPol and RNA3. Accumulation of positive- and negative-strand RNA3 and subgenomic RNA4 was detected by Northern blotting using probes specific for BMV RNA3 and RNA4. Equal loading of total RNA was verified by probing for 18S rRNA. Values represent the mean of four independent experiments.