Figure 2.

Visualisation of two tree-based assignment criteria. The distinction between a "positive" and an "ambiguous" assignment and how the assignment is achieved - based on the location of the query on the tree. A). "Liberal" - When the query barcode (Q) was sister to (Aus, Q) a clade comprising members of a single taxon, Aus, the assignment was that of the taxon in the clade, Aus (i.e. "positive"). When Q is sister to a clade comprised of multiple taxa, Aus and Bus, the assignment was "ambiguous". B). "Strict" - When the query sequence (Q) was nested within a clade comprising of members of a single taxon ((Aus, Q)Aus) the assignment was that of the taxon in the clade, Aus (i.e. "positive"). When Q is nested within a clade of multiple taxa ((Aus, Q)Bus)) the assignment was "ambiguous".