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Chondrogenic differentiation, toluidine blue staining. Chondrogenic differentiation (P3) dem-onstrated in three dimensional pellet cultures. Sulphated proteoglycan deposition (purple metachromasia) in the extracellular matrix was assessed by toluidine blue staining (a-e). Only moderate cartilage matrix formation was present in non induced cell cultures, shown for eP-MSCm (f). Scale bar = 100 μm.
