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. 2011 Aug 2;7:42. doi: 10.1186/1746-6148-7-42

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Copyright ©2011 Mensing et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RT-PCR. RT-PCR analysis for indicated genes in pellet cultures (P3) after induction of chondrogenic differentiation. Cell pellets maintained in non inductive medium were used for controls (here shown for C eP-MSCm, C eG-MSC, C eSc-MSC). Extracted mRNA from equine hyaline cartilage (ehc) was used as a positive control. Note, only eP-MSCm and eP-MSCap show expression of mRNA for collagen type 2. Length of amplicons: GAPDH 341 bp, Collagen I 219 bp, COMP 238 bp, Aggrecan 147 bp, Collagen II 159 bp.