Table 3.
Prediction of the nuclear and mitochondrial targeting sequences in tRNase ZLs from flowering plants
| Protein name | From | Nuc | Mito | Predicted mitochondrial targeting sequences |
|---|---|---|---|---|
| AcoTrz3 | AcoTrzL1 | N | - | |
| AcoTrz4 | AcoTrzL2 | - | M | MPQISSNLKLFFSKTNHSPLFQFSFKASFCSSFLLSSKTPYKPLSSVTVISSSSSSSSSSRKGPKFPPLRSRS (73) |
| AlyTrz3 | AlyTrzL1 | N | M | MINSMPYLHKNLRLLLLLSSKSSPFPLSLRPFSPRSFSLSTLFS (44) |
| AlyTrz4 | AlyTrzL2 | N | M | MLTSSMPHRHVPQNLSLFGFSPLKSSSFALFLRPFSLYPPIFASSSPSPSRRPPRTAGYRRS (62) |
| AthTrz3 | AthTrzL1 | N | M | MINSMPYLHKNLRLLRLLSSKSSPFPLSLRPFSPRSFSLSTLFS (44) |
| AthTrz4 | AthTrzL2 | N | M | MLTSSMPQNLSLFGFSPLKSSSFALILRPFSLYPPIFASSSPAPSRRPPRTAGYRRSGPSPPRRK (65) |
| BdiTrz3 | BdiTrzL1 | - | M | MPQVAAPLRLLLPLSQTLAPPAPLLHLSRRLLSFCSPASFRRAASLRALAYRRSRHPEPRRG (62) |
| CpaTrz3 | CpaTrzL1 | N | M | MFLIYPNLRLLLNPPLFLFSKPNSTPLSLFTVFASSSHKRHRSVSYRDSPFGLHRRRRNFTTFKERD (67) |
| CclTrz3 | CclTrzL1 | N | M | MPFITPNLRLLFSSSSSSLFPLKLSVPLLSTKPTNRHRSLFTILSYSKRQRSTPFPQQNQRRN (63) |
| CsiTrz3 | CsiTrzL1 | N | M | MPFITPNLRLLFSSSSSSSLFPLKLSVPLLSTKPTNRHHSLFTILSYSKRQRSTPFPQQNQRRN(64) |
| CsaTrz3 | CsaTrzL1 | N | M | MPLPHLSTLRFLFFSPSKLPFSPSLYSPKSHSLFTVLASSPPKRRRSATAPPSLNFKRRN (60) |
| EgrTrz3 | EgrTrzL1 | N | M | MPCVYSNLRLLFSSSATAAAATATAAASPFLSPLKLKLRRPSSSSSAFPLLPLPPLSSLRS (61) |
| GmaTrz3 | GmaTrzL1 | N | M | MAQVSKFGYFLLHSSLPKPSNIQFRSLLTVLASSSKRHRRK (41) |
| GmaTrz4 | GmaTrzL2 | N | M | MAQVSKFGHLLLHSSLPKPSNSNIQFRSLLTLLASSSKRHRSIPPFRRKS (50) |
| MesTrz3 | MesTrzL1 | N | M | MPQISNLRFLLSPIKPSLPFPFSKPKPYSLFTVLCSSSSSRRHRTTPNHQSLNFRSRS (58) |
| MesTrz4 | MesTrzL2 | - | M | MPNVLNFKLCLSDLLTQCCKICLQHYILTFSSLSHVLVSGKLQSSNPQTYFSINNPLPRSKNSRT (65) |
| MtrTrz3 | MtrTrzL1 | N | M | MAQILNFRNFLFLPSYKPTTHFRLRFLSTLVSSSSRRSNINAPPLHLRRRS (51) |
| MguTrz3 | MguTrzL1 | N | M | MPQSTNLRLLLSSANCHRRHPFSAASNFFPKHLSFSSSFQFFLKPQFKTREIPLLFATFSSYSKKPYATNNNSNNNNKNSRSFNRN (86) |
| OsaTrz3 | OsaTrzL1 | - | M | MPQLPSPLRRLLPLSQTLAAATPAPLLHLSRRLFSSSSSPSPSPSPRAACLRALAYRGGQAGGGGRRGHHNNLLRRG (77) |
| PtrTrz3 | PtrTrzL1 | N | M | MSHISNLRLLLSPLNPTLRFPFSSKHRPYSLLTILSSSSPYPKRRHRTTPNHPSLNFRSRS (61) |
| PpeTrz3 | PpeTrzL1 | N | M | MPQVTNLRLLFFSPFPRLSLSSLSFKPLKPRTLFTALASSYRKRHRPIPNQSPNTGARN (59) |
| SitTrz5 | SitTrzL1 | - | M | MPQVAAPLRLLLPLSQTLAPAPLLHLSRRLFTSSSPSFGRAASLRALAYRRHHHPRRGSSTLRK (64) |
| SbiTrz4 | SbiTrzL1 | N | M | MPQVAGPLRRLLPLSQTLASAPAPLLHLSRRLLSSCSPASFGRAASLRALAYRRRRHPEPRRG (63) |
| VviTrz3 | VviTrzL1 | N | M | MPHLTSFRLLYCSPLLSPFKSPFLSFSTLSKSKSPLLNPPSFFTVLSSSSGRYPKLRRHPHHLRRRN (67) |
| ZmaTrz3 | ZmaTrzL1 | N | M | MPQVAAPLRLLLPLSQTLAAPAPLLHHSRRLLSSCPASLSRAAGLRALAYRRRRHTEPRRG (61) |
The putative nuclear localization signals were predicted using PSORT http://psort.hgc.jp/form.html, while the putative mitochondrial targeting signals were predicted using MITOPROT http://ihg2.helmholtz-muenchen.de/ihg/mitoprot.html. Nuc: stand for the nucleus; Mito: mitochondria. N indicates nuclear localization and M denotes mitochondrial localization. "-", the localization could not be predicted. The numbers refer to amino acid positions starting from the N-terminus.