Table 1.
Antibody | Antigen | Cell Type | Species | Indirect Fluorescent | Tyramide Amplification | Source |
---|---|---|---|---|---|---|
RA4 | Microtubule Associated Protein (MAP) | Postmitotic Neuronal Precursors Ganglion Cell axons |
Mouse | ED3 1:500 | ED3, ED6, ED7 1:10,000 ED8-ED18 1:2000 |
Kind gift from Dr. Steven McLoon |
RMO270 | Mid-sized Neurofilament (NF-M) | Postmitotic Neuronal Precursors Adult Ganglion and Bullwhip Cells |
Mouse | ED3-ED6 1:10,000 ED7-ED18 1:5,000 |
ED6 1:20,000 ED7-ED18 1:10,000 |
Kind gift from Dr. Virgnia Lee |
3B5 | AP2α | Amacrine and Horizontal Cells | Mouse | 1:35 | N/A | Developmental Studies Hybridoma Bank |
Prox1 | Prox1 | Horizontal and Bipolar Cells | Rabbit | 1:5,000 | 1:5,000 | Millipore |
4F2 | Lim1/2 | Horizontal Cells | Mouse | 1:5 | N/A | Developmental Studies Hybridoma Bank |
67.4E12 | Lim3 | Bipolar Cells | Mouse | 1:5 | N/A | Developmental Studies Hybridoma Bank |
Visinin | Visinin | Photoreceptor Cells | Rabbit | 1:10,000 | N/A | Kind gift from Dr. Leveillard |
H5 | Vimentin | Müller Glial Cells | Mouse | 1:100 | N/A | Developmental Studies Hybridoma Bank |
39.3F7 | Islet1 | ED3-ED6: Ganglion Cells ED7 and after: All Retinal Neuronal Cells |
Mouse | 1:50 | 1:5,000 | Developmental Studies Hybridoma Bank |
Hu C/D | Hu C/D | ED3-ED6: Ganglion Cells ED7 and after: Ganglion and Amacrine Cells |
Mouse | 1:100 | N/A | Invitrogen |