Figure 1.

Western blot of supernatant from transfected 293T cells pelleted through 20% sucrose using goat anti-HIV-1 gp120 (Europe Bioproducts, UK) and mouse anti-MLV CA produced by the hybridoma cell 548 (ATCC, USA). Primary antibodies were probed with HRP-conjugated secondary antibodies and visualized by enhanced chemoluminescence (Amersham Biosciences). Band intensities were assessed by Molecular Dynamics Storm software (GE Healthcare, DK).