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. 2011 Aug 1;8:381. doi: 10.1186/1743-422X-8-381

Figure 1.

Figure 1

Western blot of supernatant from transfected 293T cells pelleted through 20% sucrose using goat anti-HIV-1 gp120 (Europe Bioproducts, UK) and mouse anti-MLV CA produced by the hybridoma cell 548 (ATCC, USA). Primary antibodies were probed with HRP-conjugated secondary antibodies and visualized by enhanced chemoluminescence (Amersham Biosciences). Band intensities were assessed by Molecular Dynamics Storm software (GE Healthcare, DK).