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. Author manuscript; available in PMC: 2011 Aug 25.
Published in final edited form as: Biol Reprod. 2001 May;64(5):1320–1330. doi: 10.1095/biolreprod64.5.1320

FIG. 1.

FIG. 1

KLC3 and ERCC2 are in close proximity. A) To establish that KLC3 and ERCC2 genes are in close proximity in the genome, PCR was employed to detect the two gene sequences in a genomic KLC3 clone (lanes 1, 4, and 7) and in high molecular weight genomic DNA (lanes 2, 5, and 8). Controls lacking DNA were also included (lanes 3, 6, and 9). For reactions in lanes 1–3, KLC3-specific primers were used (predicted product size, 300 bp). For reactions in lanes 4–6, ERCC2-specific primers were used (predicted product size, 274 bp). For reactions in lanes 7–9, primers specific for both genes, such that they span the intragenic region, were employed (predicted product size, 813 bp). B) Rat and mouse genomic DNA was compared by PCR to determine if ERCC2 and KLC3 are in close proximity in rat (lane 1), as they are in mouse (lane 2). High molecular weight genomic DNA was used as a template using primers spanning ERCC2 and KLC3 sequences as described in A. Both samples generate a product identical in size (813 bp). M, Lambda and pBluescript molecular weight markers.