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. Author manuscript; available in PMC: 2011 Aug 25.
Published in final edited form as: Biol Reprod. 2001 May;64(5):1320–1330. doi: 10.1095/biolreprod64.5.1320

FIG. 3.

FIG. 3

Interaction of KLC3 with KHC. A) To analyze KLC3 interaction with KHC, immunoprecipitations of HA-tagged KLC3 and Kif5c were conducted using anti-HA antibodies. Both proteins were in vitro transcribed and translated individually (lanes 1 and 2, respectively) or together at different ratios (lanes 3–5, increasing ratio of KHC vs. KLC3). Immunoprecipitated proteins were resolved by SDS-PAGE followed by autoradiography; KHC and KLC are indicated. B) The HR region of KLC3 was deleted, and the resulting construct, HA-tagged KLC3-ΔHR, was used in experiments similar to those described in A. The mobility of the KLC3-ΔHR mutant (lane 2) is higher than that of KLC3 (lane 1), as expected. KLC3, KLC3-ΔHR, KLC1 (positive control for association with KHC), and Mos (negative control for association with KHC) were expressed as fusion proteins with an HA-tag and co-translated with Kif5c (lanes 3–6, respectively). Complexes were immunoprecipitated with anti-HA antibodies and analyzed by SDS-PAGE.