In testis, KLC3 is predominantly expressed in spermatids. Gene expression was analyzed by RT-PCR, and total RNA was isolated from indicated tissues and elutriated male germ cells, quantified, and standardized. The cDNA was produced by random priming and amplified with primers specific for the indicated genes. Lane A: β-actin (positive control for the procedures); lane B: KLC3 (note the widespread distribution of KLC3 in various tissues, with highest KLC3 RNA expression in spermatids); lane C: ERCC2; lane D: KLC1; lane E: KLC2.