Figure 10. CA is an important determinant for HIV-1 dependence on Tnp3.

Infection assays were performed in stable HeLa Tnp3 KD cells or control cells expressing a scrambled shRNA using HIV-1_GFP vectors bearing the indicated mutation in CA. Viral stocks were normalized for RT activity and different volumes used for titrations. Infected cells were analysed by flow cytometry 48 hours post-infection. Note that the T54A and T54A/N57A have ∼20 fold lower infectivity than wild type virus. Data are representative of at least 3 independent experiments.