Table 1. tRNA mutants generated and their characteristics.

Mutant name	mutation	mutation effect
G2	G2 WT	Lysine 1,2 without CCA at 3′ end
m2	G2+TTT	G2 with TTT at 3′ end
m2a	+CCA	inhibited in nuclear import
m5	U55A	disruption of T-loop/D-loop pairing
m6	G18U/U55G	Functional revertant of m5
m10	U54A	T-loop point mutation with partial disruption of T-loop structure
m20	Lys->Glu	anticodon swap from Lysine to Glutamate
m22	Lys->Asp	anticodon swap from Lysine to Aspartate