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. 2011 Aug 25;6(8):e23736. doi: 10.1371/journal.pone.0023736

Figure 6. Msp-mediated actin rearrangement and free barbed end formation does not require PI3K activity.

Figure 6

(A) Fibroblasts were treated with or without the PI3K inhibitor LY294002 prior to Msp exposure. Cells were partially permeabilized, followed by the addition of rhodamine labelled actin monomers. Incorporation of labelled actin monomers was determined by fluorescence intensity measurement using Image J. Graph represents the mean intensity ± SEM of 3 experiments (compared to control cells, **P<0.01). (B) Representative Alexa-phalloidin stained images of total F-actin with and without LY294002 treatment prior to Msp exposure. Bar represents 25 µm.