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Fibroblasts were treated with Msp for the indicated times followed by partial permeabilization. The amount of CapZ and gelsolin released from the cell was quantified using immunoblot. Densitometry analysis of released protein is shown (mean ± SEM of 3 independent experiments, *P<0.05, **P<0.01 compared to no treatment) for gelsolin (A) and CapZ (C). Representative immunoblots are shown in panels B and D.
