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. 2011 Aug 25;6(8):e23846. doi: 10.1371/journal.pone.0023846

Figure 6. Lack of UQCRFS1/RISP expression in ρ0 cells.

Figure 6

A. Western blot analysis of RISP expression in 143B and 143B ρ0 osteosarcoma cells. Tubulin served as a loading control. B. RISP knock-down in 143B cells transduced with either pSiren (control) or shRNA directed against RISP (clone #2). C. Resistance to apoptosis was measured by analyzing SubG1 fraction by DNA staining with propidium iodide. D. In vitro invasion was measured by a Matrigel invasion assay in RISP knockdown cells. ** p<0.005 *** p<0.0005.