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. 2011 Aug 25;6(8):e23998. doi: 10.1371/journal.pone.0023998

Figure 2. Effect of thermal treatment on subpopulation of proliferating cells.

Figure 2

A. Fresh PBMC were left unstimulated (Med) or stimulated with N-Ara h 2/6 for 7 days. After harvesting, the cells were incubated with anti-CD4/CD25 antibody and afterwards stained for an anti-Ki-67 PE antibody. Data are mean percentage of Ki-67 positive cells ± SEM values for three (#65, #66 and #70) of the 12 PA subjects and seven NA controls. The percentage Ki-67+ cells was divided in the following subpopulations of cells: CD4-CD25- (dark grey bars), CD4+CD25- (white bars), CD4-CD25+ (black bars) and CD4+CD25+ (light grey) cells. B. Fresh PBMC were left unstimulated (Med) or stimulated with N-, H- or G-Ara h 2/6 for 7 days. Data are mean percentage of Ki-67 positive cells ± SEM values for three peanut-allergic patients (#65, #66 and #70) (white bars) and seven non-allergic controls (grey bars).