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. 2011 Aug 25;6(8):e24050. doi: 10.1371/journal.pone.0024050

Table 2. Primers used for reverse transcription-polymerase chain reaction (RT-PCR) analysis.

Gene Sense primer Anti-sense primer
LPL GGTCGAAGCATTGGAATCCAG TAGGGCATCTGAGAACGAGTC
PPARγ2 CCTATTGACCCAGAAAGCGATTC GCATTATGAGACATCCCCACTGC
OP CTAGGCATCACCTGTGCCATACC CAGTGACCAGTTCATCAGATTCATC
RUNX2 GTTTGTTCTCTGACCGCCTC CCAGTTCTGAAGCACCTGA
COL2A1 CCAGGACCAAAGGGACAGAAAG TTCACCAGGTTCACCAGGATTG
βIII-TUB CGAGACCTACTGCATCGACA GGGATCCACTCCACGAAGTA
NES TTCCCTTCCCCCTTGCCTAATACC TGGGCTGAGCTGTTTTCTACTTTT
AFP TGCAGCCAAAGTGAAGAGGGAAGA CATAGCGAGCAGCCCAAAGAAGAA
ALB TGCTTGAATGTGCTGATGACAGGG AAGGCAAGTCAGCAGGCATCTCATC
GAPDH ATATTGTTGCCATCAATGACC GATGGCATGGACTGTGGTCATG

LPL, lipoprotein lipase; PPARγ, peroxisome proliferator-activated receptor-γ; OP, osteopontin; RUNX2, runt-related transcription factor 2; COL2A1, α-1 type II collagen; βIII-TUB, β-tubulin III; NES, nestin; AFP, α-fetoprotein; ALB, albumin; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.