Table 2. Primers used for reverse transcription-polymerase chain reaction (RT-PCR) analysis.
Gene | Sense primer | Anti-sense primer |
LPL | GGTCGAAGCATTGGAATCCAG | TAGGGCATCTGAGAACGAGTC |
PPARγ2 | CCTATTGACCCAGAAAGCGATTC | GCATTATGAGACATCCCCACTGC |
OP | CTAGGCATCACCTGTGCCATACC | CAGTGACCAGTTCATCAGATTCATC |
RUNX2 | GTTTGTTCTCTGACCGCCTC | CCAGTTCTGAAGCACCTGA |
COL2A1 | CCAGGACCAAAGGGACAGAAAG | TTCACCAGGTTCACCAGGATTG |
βIII-TUB | CGAGACCTACTGCATCGACA | GGGATCCACTCCACGAAGTA |
NES | TTCCCTTCCCCCTTGCCTAATACC | TGGGCTGAGCTGTTTTCTACTTTT |
AFP | TGCAGCCAAAGTGAAGAGGGAAGA | CATAGCGAGCAGCCCAAAGAAGAA |
ALB | TGCTTGAATGTGCTGATGACAGGG | AAGGCAAGTCAGCAGGCATCTCATC |
GAPDH | ATATTGTTGCCATCAATGACC | GATGGCATGGACTGTGGTCATG |
LPL, lipoprotein lipase; PPARγ, peroxisome proliferator-activated receptor-γ; OP, osteopontin; RUNX2, runt-related transcription factor 2; COL2A1, α-1 type II collagen; βIII-TUB, β-tubulin III; NES, nestin; AFP, α-fetoprotein; ALB, albumin; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.