Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Aug 25;6(8):e24008. doi: 10.1371/journal.pone.0024008

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Tam et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

All eyeballs were tissue paper blotted to enable bacterial adherence to the corneal epithelium. Susceptibility to traversal by adherent bacteria was then toggled on/off by use/omission of EGTA treatment following the blotting procedure. Upper images (EGTA used to toggle on traversal) show deep bacterial traversal 6 h after bacterial challenge in EGTA-treated corneas. Lower images were PBS-treated controls (EGTA step omitted) and showed bacterial adherence to the surface without subsequent bacterial traversal. Panel (a) shows confocal technique using mice with CFP-tagged cell membranes (cyan), and panel (b) shows two-photon images of cellular NAD(P)H autofluorescence (magenta) in transgenic (C57BL/6 background) or wild-type (Black Swiss background) mouse corneas respectively. Field size: 189 µm × 189 µm.