Figure 5. Quantitative localization of bacteria within ex vivo blotted and bacterially challenged MyD88 deficient mouse corneas at 8 h time-point.

Panel (a) shows a schematic of the custom bacterial quantification program. Each image stack is divided into ∼1000 sub-volumes; in each sub-volume, the top and bottom of epithelium were localized in which 10 bins along the z-depth were assigned, followed by bacterial fluorescence quantification in each bin, and finally summation of all bins of the same z-depth across the entire image stack. Panel (b) shows quantitative analysis of bacteria traversing to the basal lamina for MyD88 (−/−) but not wild-type mice (0.00 represents the corneal surface, 1.00 the basal lamina). Average ± S.E. of three fields are shown.