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. 2011 Aug 25;6(8):e24008. doi: 10.1371/journal.pone.0024008

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Tam et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Similar to the ex vivo results at the same time-point, panels (a) and (b) show blotted corneas of MyD88 (−/−) mice were more susceptible to both bacterial adherence and traversal after 8 h of bacterial challenge as compared to blotted wild-type which showed bacterial adherence only. This observation was confirmed by quantitative localization of GFP bacteria using the custom program as shown in panel (c). Whereas in the absence of blotting, panels (d) and (e) show bacterial traversal in vivo was disabled. Only bacteria adherence was detected on MyD88 (−/−) (d) but not wild-type (e) mouse corneas. This observation was confirmed by quantitative localization of GFP bacteria using the custom program as shown in panel (f).