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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Neurochem Res. 2011 May 25;36(10):1809–1816. doi: 10.1007/s11064-011-0498-y

Figure 2.

Figure 2

Effect of route of administration and therapy delay on estrogen treatment in acute SCI animals. SCI animals were treated with low dose estrogen (10 µg/kg) via osmotic pump (p) or intravenous injection (iv) at 15 min (15), 2 hr (2), and 4 hr (4) post-injury. Neuron-specific apoptosis was assessed by double fluorescent labeling of NeuN and TUNEL (top panel). Microgliosis was measured by OX-42 staining (bottom panel). Representative images (200X) are shown from frozen sections (5 µm) in the ventral horn of caudal penumbra spinal cord tissue (n ≥ 3 animals).