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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Lab Invest. 2011 May 23;91(9):1396–1409. doi: 10.1038/labinvest.2011.73

Figure 4. MLCK activity mediates hydrogen peroxide-induced tight junction disruption.

Figure 4

NRC-1 cell monolayers were incubated with (gray) or without ML-7 (white and black bars) followed by incubation with (black and gray bars) or without (white bars) hydrogen peroxidefor 120 min. Protein extracts were immunoblotted for p-MLC and β-actin (A). TER (B) and inulin flux (C) were measured. Values are mean ± sem (n = 6). Asterisks indicate the values that are significantly (p<0.05) different from corresponding control values. Symbol, #, indicates the value that is significantly (p<0.05) different from cells treated with hydrogen peroxide in the absence of ML-7. Cell monolayers were fixed and stained for occludin and ZO-1 (D). Fluorescence images collected using a confocal microscope.