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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Lab Invest. 2011 May 23;91(9):1396–1409. doi: 10.1038/labinvest.2011.73

Figure 5. Src kinase activity mediates hydrogen peroxide-induced tight junction disruption.

Figure 5

NRC-1 cell monolayers were incubated without (white and black bars) or with genistein (dark gray bars) or PP2 (light gray bars) followed by incubation with (black bars) or without (white bars) hydrogen peroxide for 120 min. Protein extracts were immunoblotted for c-Src(pY418) and β-actin (A). TER (B) and inulin flux (C) were measured. Values are mean ± sem (n = 6). Asterisks indicate the values that are significantly (p<0.05) different from corresponding control values. Symbol, #, indicates the values that are significantly (p<0.05) different from values for cells treated with hydrogen peroxide in the absence of PP2. Cell monolayers were fixed and stained for occludin and ZO-1 (D). Fluorescence images collected using a confocal microscope.