Skip to main content
. Author manuscript; available in PMC: 2012 Sep 5.
Published in final edited form as: DNA Repair (Amst). 2011 Jul 23;10(9):934–941. doi: 10.1016/j.dnarep.2011.06.001

Figure 3. Plasmid damage assay.

Figure 3

The pET22b-lacZ DNA was isolated from wild type (Lanes 1–2) and ΔaidB (Lanes 3–4) E. coli strains grown in the absence (A) or in the presence of MNNG (5µg/ml) (B), MMS 0.04% (C), ENNG (5µg/ml) (D), digested (Lanes 2, 4) or not (Lanes 1, 3) with AlkA and AP Endo and subjected to agarose gel electrophoresis. Lane 5, 1 Kb DNA marker (NEB). OC: open circular; L: linear; SC: supercoiled.