Skip to main content
. Author manuscript; available in PMC: 2012 Sep 5.
Published in final edited form as: DNA Repair (Amst). 2011 Jul 23;10(9):934–941. doi: 10.1016/j.dnarep.2011.06.001

Figure 6. Antimutagenic activity of wild type and AidB(Δ440–541).

Figure 6

Cells were grown to a density of approximately 3 × 108 cells per ml and undiluted cultures were spread on DSEM plates ± alkylating agents and mutation frequencies determined. All mutagenesis experiments were conducted at sublethal doses of alkylating agents using strains deficient in most alkylation specific DNA repair mechanisms: aidB+ (MV6782, ada-alkBΔ25:: CmR alkA1 tag-1 aidBΔ35::TetR/pTrc99A-AidB+); aidBΔ440–541 (MV6790, ada-alkBΔ25::CmR alkA1 tag-1 aidBΔ35::TetR/pTrc99A-AidBΔ440–451); vector control (MV6780, ada-alkBΔ25::CmR alkA1 tag-1 aidBΔ35::TetR/pTrc99A).