Table 1.
Bacterial strains and plasmids
| Strains/plasmids | Description | Reference or source |
|---|---|---|
| Strains | ||
| MG1655 | wild-type; F− λ− ilvG rfb50 rph1 | [38] |
| MV5924 | aidBΔ::TetR derivative of MG1655 in which the aidB gene is replaced by a tetracycline resistance cassette using the methods of Murphy and Campellone [39] | [11] |
| MV6774 | ada-alkBΔ25::CmR alkA1 tag-1 aidBΔ35::TetR derivative of MV1161 [13] | This study |
| MV6780 | ada-alkBΔ25::CmR alkA1 tag-1 aidBΔ35::TetR/pTrc99A) | This study |
| MV6782 | ada-alkBΔ25::CmR alkA1 tag-1 aidBΔ35::TetR/pMV435 (pTrc99A-AidB+) | This study |
| MV6790 | ada-alkBΔ25::CmR alkA1 tag-1 aidBΔ35::TetR/pMV1526 (pTrc99A-AidBΔ440– 451) | This study |
| Plasmids | ||
| pET22b(+) | carries an N-terminal pelB signal sequence for potential periplasmic localization, plus an optional C-terminal His-tag sequence | This study |
| pET22b-aidB | pET22bΔ(NdeI-HindIII)Ω(aidB gene) | This study |
| pET22b-lacZ | pET22bΔ(HindIII-XhoI)Ω(lacZ gene) | This study |
| pET22b-PrrnB(+UP)-lacZ | pET22b-lacZΔ(SphI-HindIII)ΩPrrnB(+UP) | This study |
| pET22b-PrrnB(−UP)-lacZ | pET22b-lacZΔ(SphI-HindIII)ΩPrrnB(−UP) | This study |
| pET22b-PleuA-lacZ | pET22b-lacZΔ(SphI-HindIII)ΩPleuA | This study |
| pET22b-PompF-lacZ | pET22b-lacZΔ(SphI-HindIII)ΩPompF | This study |
| pTrc99A | E. coli expression vector | [10] |
| pMV435 | pTrc99A-AidB+ | [10] |
| pMV1526 | pTrc99A-AidBΔ440–451 | This study |