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. Author manuscript; available in PMC: 2012 Sep 15.

Published in final edited form as: Mol Cell Endocrinol. 2011 Jul 8;344(1-2):25–34. doi: 10.1016/j.mce.2011.04.011

(A) Dye diffusion studies to assess functional gap junctions. Donor ESC doubly labeled with gap junction permeable (calcein, green) and impermeable (diI, red) dyes were cultured with unlabeled recipient ESC as described in Materials and Methods. White arrows indicate diffusion of calcein from donor to recipient cells. Similarities in confluence of the variously treated cultures is demonstrated by phase contrast (phase) microscopy in the lower panel. Original magnification × 200. (B) Quantification of the number of gap junctions (indicated by calcein diffusion, above) per high power field (HPF) indicates that 18α-glycyrrhetinic acid (AGA) inhibited gap junction coupling under control and hormone-treated conditions. In addition, 4 days of E/P/c treatment resulted in a significant increase in functional coupling compared to control (*P<0.05, t-test). The figure reflects a selected representative experiment.

(A) Dye diffusion studies to assess functional gap junctions. Donor ESC doubly labeled with gap junction permeable (calcein, green) and impermeable (diI, red) dyes were cultured with unlabeled recipient ESC as described in Materials and Methods. White arrows indicate diffusion of calcein from donor to recipient cells. Similarities in confluence of the variously treated cultures is demonstrated by phase contrast (phase) microscopy in the lower panel. Original magnification × 200. (B) Quantification of the number of gap junctions (indicated by calcein diffusion, above) per high power field (HPF) indicates that 18α-glycyrrhetinic acid (AGA) inhibited gap junction coupling under control and hormone-treated conditions. In addition, 4 days of E/P/c treatment resulted in a significant increase in functional coupling compared to control (*P<0.05, t-test). The figure reflects a selected representative experiment.