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Inhibitory effects of AGA on VEGF production are partly mediated at the level of mRNA metabolism. Real-time RT-PCR was used to quantify VEGF mRNA transcripts in decidualized ESC. Incubation with E/P/c for 8 days in the presence of AGA resulted in ~50% reduction in steady-state VEGF mRNA. The inset reveals the PCR amplification profiles and melting curve analyses, showing precise, single peaks for the amplified cDNAs.
