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. Author manuscript; available in PMC: 2011 Aug 26.
Published in final edited form as: Cell Stem Cell. 2010 Jun 4;6(6):535–546. doi: 10.1016/j.stem.2010.05.003

Table 1.

Introduction of transgenes into human ES cells and hLR5 cells by electroporation

Experiment Cell type # Cells per electroporation # electroporations Size of the construct Drug selection Average # colonies per 10 × 106 cells
#1 Human ES 1 × 107 10 20 kbase Hygromycin 6.8
#2 Human ES 1 × 107 10 20 kbase Hygromycin 10.6
#3 Human ES 1 × 107 6 20 kbase Hygromycin 12
#4 Human ES 5 × 106 3 14 kbase Puromycin 5.7
#5 hLR5 1 × 107 2 20 kbase Hygromycin ≫1500
#6 hLR5 1 × 107 2 20 kbase Hygromycin ≫1500
#7 hLR5 1 × 107 1 14 kbase Puromycin 1300
#8 hLR5 1 × 107 1 14 kbase Puromycin 1100