Table 1.
Experiment | Cell type | # Cells per electroporation | # electroporations | Size of the construct | Drug selection | Average # colonies per 10 × 106 cells |
---|---|---|---|---|---|---|
#1 | Human ES | 1 × 107 | 10 | 20 kbase | Hygromycin | 6.8 |
#2 | Human ES | 1 × 107 | 10 | 20 kbase | Hygromycin | 10.6 |
#3 | Human ES | 1 × 107 | 6 | 20 kbase | Hygromycin | 12 |
#4 | Human ES | 5 × 106 | 3 | 14 kbase | Puromycin | 5.7 |
#5 | hLR5 | 1 × 107 | 2 | 20 kbase | Hygromycin | ≫1500 |
#6 | hLR5 | 1 × 107 | 2 | 20 kbase | Hygromycin | ≫1500 |
#7 | hLR5 | 1 × 107 | 1 | 14 kbase | Puromycin | 1300 |
#8 | hLR5 | 1 × 107 | 1 | 14 kbase | Puromycin | 1100 |