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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Immunol. 2011 Feb 23;186(7):3847–3857. doi: 10.4049/jimmunol.1000361

FIGURE 1.

FIGURE 1

Screening of overlapping 15-aa peptides spanning the entire length of the Her-2/neu protein with neu-specific CD8+ T cells from vaccinated neu-N mice identifies six new epitopes. Splenocytes from five vaccinated neu-N mice were pulsed with each peptide pool or a single peptide for 1 wk. CD8+ T cells were isolated and incubated with T2-Dq cells pulsed with the corresponding peptide(s) or the irrelevant LCMV nucleoprotein peptide NP118–126. A, Detection of GM-CSF secretion by neu-specific CD8+ T cells after stimulation with peptide pools containing 10 overlapping 15-aa peptides. B, Individual 15-aa peptides elicit responses from neu-specific CD8+ T cell repertoire that correlate with the peptide pool data. Plotted are the mean GM-CSF ELISA values for five mice after background NP118–126 levels were subtracted.