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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: J Immunol. 2011 Feb 23;186(7):3847–3857. doi: 10.4049/jimmunol.1000361

FIGURE 4.

FIGURE 4

Lower relative avidities are detected among nondominant CD8+ T cell responses when compared with RNEU420–429 T cell responses in FVB/N and neu-N mice. A and B, RNEU420–429-specific responses are the most abundant and avid in FVB/N mice. C and D, 24F- and 24F1-specific responses are the most avid in neu-N mice. Lymphocytes were isolated 14 d after tumor challenge from 10 tumor-bearing mice treated with the 3T3neuGM vaccine and stained with increasing dilutions of H-2Dq/peptide tetramer and anti-CD8 straight ex vivo. Tetramer dilutions were made from 1 mg/ml tetramer stock solutions. Graphed data depict the percentage of CD8+ T cells that are tetramer positive after subtraction of background tetramer staining with H-2Dq/NP118–126. These experiments were repeated at least twice with similar results.