Table 1.

Mean number (± SD) of labelled neurons in the Amb and 10N following application of tracer to either the SLN or RLN

	Amb			10N	SolI
	Fluorescence	Bright-field	Total	Bright-field	Fluo/Bright
SLN	112 ± 24	122 ± 40	119 ± 36	75 ± 25	Yes
RLN	159 ± 28	152 ± 35	155 ± 30	–	No

For the Amb the table shows the number of neurons counted either with bright-field microscopy or following fluorescent labelling of the nucleus with DAPI. Both methods yield similar results and so the counts were combined to obtain the numbers shown in the ‘Total’ column of the table. It was not possible to employ the fluorescent-counting method for labelled neurons in 10N, and therefore only the bright-field method was employed. The table also records the presence or absence of labelled fibres in the SolI following labelling of each of the nerves.

10N, dorsal motor nucleus of the vagus nerve; Amb, nucleus ambiguus; RLN, recurrent laryngeal nerve; SLN, superior laryngeal nerve; SolI, interstitial nucleus of the solitary tract.