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. 2009 Apr 29;29(17):5525–5535. doi: 10.1523/JNEUROSCI.5469-08.2009

Figure 3.

Figure 3.

Nmnat axonal protective activity is conserved throughout evolution. A, The Nmnat enzymatic activities from HEK293T cell lysates expressing EGFP, Nmnat1, dNmnat, yNmnat, or mjNmnat demonstrated that each enzyme had similar NAD+ synthetic activity. Error bars represent SD. B, The rate of axonal degeneration after injury was quantified in DRG neurons expressing EGFP, mouse Nmnat1, dNmnat, yNmnat, or mjNmnat. Twelve fields were evaluated for each condition, and each experiment was repeated three times. The degeneration index value ± SD at 24, 48, and 72 h is displayed. All Nmnat enzymes, from mammalian to Archaea, delayed axonal degeneration of injured DRG neurons in a comparable manner. Nmnat1-, dNmnat-, yNmnat-, or mjNmnat- versus EGFP-expressing neurons, p < 0.001 (n = 3).