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. 2009 Apr 29;29(17):5525–5535. doi: 10.1523/JNEUROSCI.5469-08.2009

Figure 6.

Figure 6.

Neurons expressing Nampt siRNA have low levels of NAD+, but Nmnat-mediated axonal protection is intact. A, DRG neurons were infected with lentiviruses expressing EGFP or cytNmnat1 as well as siRNAs targeting Nampt or luciferase. After 8 d, NAD+ levels were measured using HPLC. Both Nampt siRNAs effectively reduced NAD+ levels. Overexpression of cytNmnat1 had no effect on NAD+ levels. Values are from three independent experiments; *p < 0.001 versus luciferase siRNA-expressing neurons. Error bars represent SD. B, Axonal degeneration assays were performed using neurons infected with lentiviruses expressing the indicated siRNA and either cytNmnat1 or EGFP, and degeneration indices were calculated. Sixteen fields were evaluated for each condition, and each experiment was repeated three times. The degeneration index value ± SD at 0, 24, 48, and 72 h is displayed. *Significant difference (p < 0.001) between cytNmnat1- and EGFP-expressing neurons expressing either luciferase or Nampt siRNA. Note that Nampt siRNA had no effect on Nmnat-mediated axonal protection.