Table 1.
Kinetic parameters of purified recombinant Nmnat1 proteins
| Enzyme | Substrate | Km (μm) | Vmax (μmol · min−1 · μg−1) | kcat (s−1) |
|---|---|---|---|---|
| Nmnat1 | ATP | 50.4 | 8.1 | 4.5 |
| NMN | 27.6 | 9.9 | 5.5 | |
| Nmnat1(W170A) | NMN | 1.4 × 103 | 0.0037 | 0.0021 |
| Nmnat1(H24A) | ATP | 47.3 | 0.024 | 0.013 |
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