Fig. 3.

Effects of RAD001, TAX, and ZOL alone and in combination on tumor and bone. Bone histomorphometry was used to evaluate the effects of the treatment on tissue volume (TV) and tumor volume (TuV). Analyses were performed on longitudinal sections of tibiae (n = 5 per group). BMD and BHM analyses were used to evaluate the effects of the treatments on bone. Tissue volume (A), and tumor volume (B) were significantly decreased in all treatment groups versus the control group. The largest decreases in TV and TuV were observed with all three agents in combination. Comparison of BMD normalized to contralateral (non-tumored) tibiae for each treatment. C4-2 cells cause decreases in BMD. Treatment with low doses of ZOL alone or in combination preserved bone (C). Treatments with ZOL generally caused increases in BV. RAD001 treatment increased BV, but the combination of three agents did not (D). Results are plotted as mean ± SEM. Significant differences versus controls are denoted by: *P < 0.05, **P < 0.01.