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. 2011 Sep;17(9):1678–1687. doi: 10.1261/rna.2592411

FIGURE 2.

FIGURE 2.

Changes in YrdC fluorescence with the addition of differentially modified ASLs. (A) The titration of YrdC with unmodified ASLLysUUU, ASLVal3UAC, and yeast ASLPheGAA. (B) The titration of YrdC with the unmodified ASLLysUUU and the t6A37-modified ASLLysUUU-t6A37. (C) The titration of YrdC with nucleoside pentamers analogous to the tRNALys anticodon region, U33UUUA37, and U33UUUt6A37. (D) The titration of YrdC with variously modified ASLLysUUU with the modifications 5-methylaminomethyluridine, mnm5U34, 2-thiouridine, s2U34, pseudouridine, Ψ39, and the 2-methylthio derivative of t6A37, ms2t6A37.