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Nucleoside A37 monitored for base flipping. (A) The nucleoside sequence of the 2-aminopurine ribonucleoside (2AP37) construct. (B) Fluorescence changes in the tryptophans of YrdC with addition of the unmodified ASLLysUUU and the 2AP37-modified ASLLysUUU-2AP37. (C) Changes in fluorescence of 2AP37 (λex = 310 nm; λem = 375 nm) with addition of increasing concentrations of YrdC.
