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PAPD5 adds poly(A) tails to the unmodified in vitro transcript of S. cerevisiae tRNAiMet, but not to the native tRNA isolated from yeast. PAPD5 protein expressed in E. coli (PAPD5 rec.) or in HEK293 cells was incubated with the different RNA substrates in the presence of ATP, and the reactions were stopped after the reaction times indicated (0, 10, 20, 40, 60 min).
