Figure 5.

Inefficient recognition of the L₂₆₈M mutant by public TRBV4-3/TRBJ1-3 clonotypes. (A) In vivo dominance of public TRBV4-3/TRBJ1-3 clonotypes and prevalent viral variants at the time of sampling. *Codominant viral KK10 sequences. (B) Ag sensitivity of representative CD8⁺ T-cell clones for the wt KK10 and L₂₆₈M mutant epitopes was measured in Cr⁵¹ cytotoxicity assays using EBV-transformed HLA-B*2705⁺ B cells pulsed with the corresponding peptides across a range of concentrations. (C) Suppression of HIV replication in HLA-B*2705⁺ CD4⁺ T cells infected with wt (HIV_NL4-3) virus (■) or a mutant virus encoding the L₂₆₈M epitope mutation () by representative CD8⁺ T-cell clones at 3 different E:T ratios. The percentage of p24⁺ CD4⁺ T cells was measured by flow cytometry at day 3 postinfection.