FIGURE 1.
Effect of ZnCl2 on the binding of HRG to γA/γA- or γA/γ′-fibrinogen. γA/γA-Fibrinogen (triangles) or γA/γ′-fibrinogen (circles) was immobilized to 6000–7000 RU on separate flow cells of a CM4 BIAcore chip. An unmodified flow cell served as control. HRG (0.2 μm) was injected into flow cells in the absence or presence of ZnCl2 at the concentrations indicated. The RU at equilibrium (Req) was calculated and, after background correction, is plotted against the input ZnCl2 concentrations. Data represent the mean ± S.D. of two experiments, and lines represent nonlinear regression analyses of the data.