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Effect of the γ′-peptide-directed IgG on HRG binding to γA/γA- or γA/γ′-fibrinogen. Flow cells containing immobilized γA/γA-fibrinogen (triangles) or γA/γ′-fibrinogen (circles) were pretreated with (closed) or without (open) 0.5 μm γ′-peptide-directed IgG before injection of HRG (0–1.0 μm). Symbols represent the mean ± S.D. of two experiments, and lines represent nonlinear regression analyses of the data.
