FIGURE 6.

Dissociation of ¹²⁵I-HRG from preformed γ_A/γ_A- or γ_A/γ′-fibrin clots. Aliquots of 5 μm γ_A/γ_A- (A) or γ_A/γ′-fibrinogen (B) containing 2 mm CaCl₂, 20 μm ZnCl₂, 20 nm factor XIII, and 50 nm ¹²⁵I-HRG were clotted with 10 nm thrombin around plastic inoculation loops. After incubation at 23 °C for 45 min, clots were counted for radioactivity and incubated in tubes containing 5 ml of 2 m NaCl and 2 mm of EDTA (triangles), 2 mm CaCl₂ and 10 μm diethyldithiocarbamate trihydrate (squares), or 2 mm CaCl₂ and 20 μm ZnCl₂ (circles). At intervals, clots were removed, and residual radioactivity was used to determine the percent of ¹²⁵I-HRG that remained clot-associated. The symbols represent the mean ± S.D. of three experiments, whereas the lines represent nonlinear regression analyses of the data using two-component exponential decay model.