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. 2011 Jul 7;286(35):30384–30392. doi: 10.1074/jbc.M111.262899

FIGURE 2.

FIGURE 2.

Representative budding yeast Sc-MyoII (Myo1p) and Sc-MyoV (Myo2p) isolations. A, Sc-MyoII and B, Sc-MyoV were isolated from budding yeast lysates using glutathione-Sepharose following co-overexpression of heavy chain and GST-tagged light chains (Mlc1p and Mlc2p for Sc-MyoII; Mlc1p and Cmd1p/calmodulin for Sc-MyoV). Numbers to the left of the SDS-PAGE gel indicate molecular weight markers (kDa).