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PAF modulates the patterns of phosphorylation in ECV-304 cells transfected with different targeted eNOS constructs. Left panels, Western blots; right panels, quantification. A, phosphorylation of ECV-eNOS-GFP cells at Ser-1177 and Thr-495 (n = 3). B, phosphorylation (p) of ECV-GFP-eNOS-G2A cells at Ser-1177 and Thr-495 (n = 4). C, phosphorylation of ECV-GFP-eNOS-CAAX cells at Ser-1177 and Thr-495 (n = 3). *, p < 0.05 compared with the control (C).
