FIGURE 5.

Association of eNOS and sGC. A, experiments in ECV-eNOS-GFP cells. Samples were immunoprecipitated with either anti-eNOS antibody (IP) or nonspecific IgG (negative control (Neg Ctl)) and subsequently blotted for sGC. IP blots correspond to samples obtained after the pulldown process, whereas Input blots correspond to samples before immunoprecipitation and serve as a loading reference. PAF stimulated the time-dependent association of eNOS and sGC in ECV-eNOS-GFP cells. B, experiments in ECV-304 cells that lack eNOS and served as a second negative control for eNOS and as a test for the specificity of anti-eNOS antibody. Samples were immunoprecipitated with anti-eNOS antibody and blotted against sGC. C, control without PAF. n = 3 in both panels.